Journal: Cell Death & Disease

Article Title: In situ generated D-peptidic nanofibrils as multifaceted apoptotic inducers to target cancer cells

doi: 10.1038/cddis.2016.466

Figure Lengend Snippet: Pericellular D-peptide nanofibrils selectively inhibit cancer cells in co-culture via presenting autocrine death ligands, and act as a multifaceted death signal of the apoptosis of cancer cells. Western blot shows the difference of death ligands ( a ) in pericellular DTP nanofibrils and conditioned medium (CM) with HeLa cells; ( b ) in four kinds of cell lysates (i.e., HeLa, MES-SA/Dx5, T98G, A2780-cis). ( c ) Fluorescent images show the expression of cell death ligands. Blue indicates all the live cells stained by Hoechst 33342; and green indicates the secondary antibodies. The initial number of cells is 1.0 × 10 5 /well. The scale bar is 100 μ m for low magnification (up) and 50 μ m for high magnification (down). ( d ) The quantification of integrated density of fluorescence of HeLa cells showed in c . Western blot shows change of relative amount of ( e ) cell death receptors; ( f ) down-stream proteins over time in HeLa cells treated by 300 μ M of pDTP. ( g ) Western blot shows the difference of PLAP expressed on membrane of three kinds of cells (i.e., HeLa, HS-5, HepG2). ( h ) Relative cell viability of the co-cultured cells (HS-5 and MDR cancer cells) incubated with pDTP in DMEM for 48 h. The initial number of cells is 1.0 × 10 4 /well (e.g., 1.0 × 10 4 MDR or HS-5 cells, or mixture of 5.0 × 10 3 MDR cells and 5.0 × 10 3 HS-5 cells). ( i ) Relative cell viability of MDR cells treated with pDTP and zVAD-fmk for 48 h. Relative cell viability of MDR cells treated with 400 or 500 μ M pDTP and ( j ) TNF- α -related mAbs; ( k ) TRAIL-related mAbs; or ( l ) CD95L-related mAbs for 48 h. The initial number of cells is 1.0 × 10 4 /well. n =3. Data are shown as mean±S.D. * P <0.05, ** P <0.01 by Student's t -test

Article Snippet: HeLa, T98G, HS-5, HepG2 cells are purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), MES-SA/Dx5 and A2780-cis cells from Sigma-Aldrich Co. (St. Louis, MO, USA), and HeLa-GFP cell from Cell Biolabs, Inc. (San Diego, CA, USA) HeLa, T98G, HeLa-GFP, HepG2 cells are propagated in minimum essential media (MEM, Invitrogen Life Technologies); HS-5 cell is cultured in Dulbecco's modified Eagle's medium (DMEM, Invitrogen Life Technologies, Waltham, MA, USA); MES-SA/Dx5 cell is propagated in McCoy's 5A (Invitrogen Life Technologies); A2780-cis cell is propagated in RPMI-1640 medium (ATCC).

Techniques: Co-Culture Assay, Western Blot, Expressing, Staining, Fluorescence, Cell Culture, Incubation

Journal: Cell Death & Disease

Article Title: In situ generated D-peptidic nanofibrils as multifaceted apoptotic inducers to target cancer cells

doi: 10.1038/cddis.2016.466

Figure Lengend Snippet: The summary of the receptor/ligand pairs required for pDTP toxicity in different cell lines (↑ indicates increasing the cytotoxicity;↓indicates decreasing the cytotoxicity; − indicates little effect)

Article Snippet: HeLa, T98G, HS-5, HepG2 cells are purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), MES-SA/Dx5 and A2780-cis cells from Sigma-Aldrich Co. (St. Louis, MO, USA), and HeLa-GFP cell from Cell Biolabs, Inc. (San Diego, CA, USA) HeLa, T98G, HeLa-GFP, HepG2 cells are propagated in minimum essential media (MEM, Invitrogen Life Technologies); HS-5 cell is cultured in Dulbecco's modified Eagle's medium (DMEM, Invitrogen Life Technologies, Waltham, MA, USA); MES-SA/Dx5 cell is propagated in McCoy's 5A (Invitrogen Life Technologies); A2780-cis cell is propagated in RPMI-1640 medium (ATCC).

Techniques:

Journal: Cell Death & Disease

Article Title: In situ generated D-peptidic nanofibrils as multifaceted apoptotic inducers to target cancer cells

doi: 10.1038/cddis.2016.466

Figure Lengend Snippet: Pericellular D-peptide nanofibrils affect the expression of death receptors and selectively inhibit MDR cancer cells for combination therapy. Western blot shows change of relative amount of cell death receptors and two down-stream proteins over time in ( a ) MES-SA/Dx5, ( b ) T98G, ( c ) A2780-cis, or ( d ) HS-5 cells treated by pDTP. ( e ) Relative cell viability of the co-culture of A2780-cis and HS-5, A2780-cis, and HS-5 cells incubated with cisplatin (CDDP) at the concentration of 6 μ g/ml in DMEM. ( f ) Relative cell viability of A2780-cis cells incubated with 216 μ g/ml pDTP, 15 μ g/ml CDDP, or the mixture of 216 μ g/ml pDTP and 15 μ g/ml CDDP for 24 h. Cells are pretreated with CDDP for 12 h. Relative cell viability of HeLa cells incubated with ( g ) pDTP of different concentrations and 10 or 20 μ M BAY 11-7085 or 500 nM BTZ; ( h ) BAY of different concentrations and 100 μ M pDTP. The incubation time is 48 h. The initial number of cells is 1.0 × 10 4 /well. n =3. Data are shown as mean±S.D. * P <0.05, ** P <0.01 by Student's t -test

Article Snippet: HeLa, T98G, HS-5, HepG2 cells are purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), MES-SA/Dx5 and A2780-cis cells from Sigma-Aldrich Co. (St. Louis, MO, USA), and HeLa-GFP cell from Cell Biolabs, Inc. (San Diego, CA, USA) HeLa, T98G, HeLa-GFP, HepG2 cells are propagated in minimum essential media (MEM, Invitrogen Life Technologies); HS-5 cell is cultured in Dulbecco's modified Eagle's medium (DMEM, Invitrogen Life Technologies, Waltham, MA, USA); MES-SA/Dx5 cell is propagated in McCoy's 5A (Invitrogen Life Technologies); A2780-cis cell is propagated in RPMI-1640 medium (ATCC).

Techniques: Expressing, Western Blot, Co-Culture Assay, Incubation, Concentration Assay

Journal: Cell Death & Disease

Article Title: In situ generated D-peptidic nanofibrils as multifaceted apoptotic inducers to target cancer cells

doi: 10.1038/cddis.2016.466

Figure Lengend Snippet: Pericellular D-peptide nanofibrils selectively inhibit MES-SA/Dx5 tumor on nu/nu mice. ( a ) Tumor progression curves of mice bearing MES-SA/Dx5 tumors. In all, 0.1 ml of pDTP at 1.2 mg/kg, 12 mg/kg, or just PBS buffer as control was injected subcutaneously and peritumorally in every 3 days (five doses, starting day 1). Data are shown as mean±S.D. (each group contains six mice). * P <0.05, ** P <0.01 by Student's t -test. F values of every 3 days by Anova statistic with the critical value F =3.68. The change of the ( b ) body weights or ( c ) spleen or thymus index of nu/nu mice during the treatment. In all, 0.1 ml of pDTP at 10 mg/ml, 20 mg/ml, or just PBS buffer as control was injected intravenously (starting day 0), and then the body weights or spleen or thymus index of mice were tested. ( d ) Hematoxylin and eosin (H&E) staining images of heart, spleen, kidney, liver, or lung from the nu/nu mice treated with 0.1 ml of pDTP at 20 mg/ml, or just PBS buffer as control by intravenous injection. The scale bar is 100 μ m

Article Snippet: HeLa, T98G, HS-5, HepG2 cells are purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), MES-SA/Dx5 and A2780-cis cells from Sigma-Aldrich Co. (St. Louis, MO, USA), and HeLa-GFP cell from Cell Biolabs, Inc. (San Diego, CA, USA) HeLa, T98G, HeLa-GFP, HepG2 cells are propagated in minimum essential media (MEM, Invitrogen Life Technologies); HS-5 cell is cultured in Dulbecco's modified Eagle's medium (DMEM, Invitrogen Life Technologies, Waltham, MA, USA); MES-SA/Dx5 cell is propagated in McCoy's 5A (Invitrogen Life Technologies); A2780-cis cell is propagated in RPMI-1640 medium (ATCC).

Techniques: Injection, Staining

Journal: Pharmaceutics

Article Title: Olaparib Conjugates with Selenopheno[3,2- c ]quinolinone Inhibit PARP1 and Reverse ABCB1-Related Multidrug Resistance

doi: 10.3390/pharmaceutics14122571

Figure Lengend Snippet: Cytotoxicity of 5a – c on the MES-SA/Dx-5 cell line.

Article Snippet: MES-SA (Human uterine sarcoma, ATCC ® CRL-1976TM) and H9C2 (rat cardiomyocytes, ATCC CRL-1446TM), MCF-7 (human adenocarcinoma, ATCC HTB-22TM), and HCC1937 (human breast carcinoma, ATCC CRL-2336TM) cell lines were obtained from American Type Culture Collection, and the MES-SA/Dx-5 (doxorubicin resistant human uterine sarcoma with high levels of MDR1 mRNA and P-glycoprotein, ECACC 95051031-1VL) cell line was obtained from the European Collection of Authenticated Cell Cultures.

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